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Genechem puromycin revg1001
Puromycin Revg1001, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/puromycin+revg1001/and/pmc13141025-326-2-13
Average 86 stars, based on 1 article reviews
puromycin revg1001 - by Bioz Stars, 2026-10
86/100 stars

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Article Title: Sinomenine inhibits macrophage M1 polarization by downregulating α7nAChR via a feedback pathway of α7nAChR/ERK/Egr-1.
Article Snippet: Background: Sinomenine (SIN) is an anti-inflammatory drug that has been used for decades in China to treat arthritis.. In a previous study, SIN acted on α7 nicotinic acetylcholine receptor (α7nAChR) to inhibit inflammatory responses in macrophages, which indicates a new anti-inflammatory mechanism of SIN.. However, the level of α7nAChR was increased in the inflammatory responses and was downregulated by SIN in vitro, so the underlying mechanisms of SIN acting on α7nAChR remain unclear.

Article Title: MicroRNA let-7i inhibits granulosa-luteal cell proliferation and oestradiol biosynthesis by directly targeting IMP2.
Article Snippet: Research question: Increased granulosa cell division is associated with abnormal folliculogenesis in polycystic ovary syndrome (PCOS).. Lethal-7i microRNA (let-7i) may play an important role in the follicular development and granulosa cell growth; therefore is let-7i involved in PCOS pathogenesis?. Design: The expression of let-7i was measured in granulosa-luteal cells (GLC) from women with or without PCOS.

Article Title: Pterostilbene Induces Apoptosis in Awakening Quiescent Prostate Cancer Cells by Upregulating C/EBP-β-Mediated SOD2 Transcription.
Article Snippet: Cells expressing high levels of GFP were selected with 0.2 mg/ml puromycin (REVG1001; GeneChem, Shanghai, China).

Article Title: GUTK induces apoptosis in reactivating quiescent prostate cancer cells via Aurora A-mediated stabilization of SOD2
Article Snippet: PCa cells were maintained in RPMI 1640 medium (MB4374, Meilun Biotechnology, Dalian, China) supplemented with 10% fetal bovine serum (FBS, 04-001-1 ACS, Biological Industries, Beijing, China).

Article Title: ISIR and its human homolog gene AK131315 strengthen LPS-induced inflammation and acute lung injury by promoting TAK1-dependent NF-κB and MAPK signaling.
Article Snippet: Acute lung injury (ALI), a critical complication observed in various clinical disorders, is characterized by widespread inflammation, neutrophil infiltration, and proinflammatory cytokine production.. This study showed that the recently identified non-coding RNA ISIR and its human homolog gene AK131315 played a role in regulating lipopolysaccharide (LPS)-induced inflammatory responses.. ISIR and AK131315 increased the production of inflammatory cytokines in LPS-stimulated macrophages, and exogenous ISIR aggravated LPS-induced lung inflammation in an animal model of ALI.

Transfection:

Article Title: GLUT1 sensitizes tumor cells to EGFR-TKIs by binding with activated EGFR and regulating its downstream signaling pathways.
Article Snippet: Stable cell line Building Lentivirus FLAG-vector (Ubi-MCS-3FLAG-CBh-gcGFPIRES-puromycin), FLAG-EGFRvIII (delete 30–296 aa), or FLAG-EGFRvI (delete 25–645 aa), all purchased by Shanghai Genechem Co., Ltd. H1299 and 293T cells were transfected with lentiviral particles for 48 h, then incubated in fresh medium. .. 2 μg/mL Puromycin (REVG1001, Genechem) was used to build the transfected stable cell lines. ..

Article Title: Establishment of Canine Oral Mucosal Melanoma Cell Lines and Their Xenogeneic Animal Models
Article Snippet: .. Then, the stable transplants were screened with Puromycin (REVG1001, Genechem) for 48 h. Cells that were not successfully transfected after 48 h died, and the remaining cells were screened by reducing the concentration of Puromycin to the maintenance concentration (1/2~1/4 of the screening concentration) so as to continue the screening and amplification of the infected cells. ..

Stable Transfection:

Article Title: GLUT1 sensitizes tumor cells to EGFR-TKIs by binding with activated EGFR and regulating its downstream signaling pathways.
Article Snippet: Stable cell line Building Lentivirus FLAG-vector (Ubi-MCS-3FLAG-CBh-gcGFPIRES-puromycin), FLAG-EGFRvIII (delete 30–296 aa), or FLAG-EGFRvI (delete 25–645 aa), all purchased by Shanghai Genechem Co., Ltd. H1299 and 293T cells were transfected with lentiviral particles for 48 h, then incubated in fresh medium. .. 2 μg/mL Puromycin (REVG1001, Genechem) was used to build the transfected stable cell lines. ..

Concentration Assay:

Article Title: Establishment of Canine Oral Mucosal Melanoma Cell Lines and Their Xenogeneic Animal Models
Article Snippet: .. Then, the stable transplants were screened with Puromycin (REVG1001, Genechem) for 48 h. Cells that were not successfully transfected after 48 h died, and the remaining cells were screened by reducing the concentration of Puromycin to the maintenance concentration (1/2~1/4 of the screening concentration) so as to continue the screening and amplification of the infected cells. ..

Amplification:

Article Title: Establishment of Canine Oral Mucosal Melanoma Cell Lines and Their Xenogeneic Animal Models
Article Snippet: .. Then, the stable transplants were screened with Puromycin (REVG1001, Genechem) for 48 h. Cells that were not successfully transfected after 48 h died, and the remaining cells were screened by reducing the concentration of Puromycin to the maintenance concentration (1/2~1/4 of the screening concentration) so as to continue the screening and amplification of the infected cells. ..

Infection:

Article Title: Establishment of Canine Oral Mucosal Melanoma Cell Lines and Their Xenogeneic Animal Models
Article Snippet: .. Then, the stable transplants were screened with Puromycin (REVG1001, Genechem) for 48 h. Cells that were not successfully transfected after 48 h died, and the remaining cells were screened by reducing the concentration of Puromycin to the maintenance concentration (1/2~1/4 of the screening concentration) so as to continue the screening and amplification of the infected cells. ..

Article Title: Nuclear translocation of metabolic enzyme PKM2 participates in high glucose-promoted HCC metastasis by strengthening immunosuppressive environment
Article Snippet: .. The lentivirus (MOI = 20) was added to the medium along with a lentivirus infection enhancement solution (4% HitransG P, GeneChem, REVG005) and incubated with cells for 16 h. Then, the infection solution was substituted with regular complete medium and the cells were further incubated for 48–72 h. These cells were subsequently selected using puromycin (2.5 μg/mL, GeneChem, REVG1001). ..

Incubation:

Article Title: Nuclear translocation of metabolic enzyme PKM2 participates in high glucose-promoted HCC metastasis by strengthening immunosuppressive environment
Article Snippet: .. The lentivirus (MOI = 20) was added to the medium along with a lentivirus infection enhancement solution (4% HitransG P, GeneChem, REVG005) and incubated with cells for 16 h. Then, the infection solution was substituted with regular complete medium and the cells were further incubated for 48–72 h. These cells were subsequently selected using puromycin (2.5 μg/mL, GeneChem, REVG1001). ..



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